Medion Diagnostics - Rhesus Sera


			Medion Diagnostics AG CH-3186 Düdingen

Product list

Rhesus Sera

Complete range of monoclonal Rhesus sera

IgM Anti-D (MS-201) Mono-Type^®
Contains a human monoclonal IgM antibody, which allows for the detection of all D variants with the exception of category D^VI.

Field trials have shown that the D antigen associated with Rh33 is detected as well as most cases of a weak D expression (D^u). Due to its high minimum titer, tube tests can be used as immediate spin. Slide tests are incubated for 5 minutes at room temperature.

Combi Anti-D Mono-Type^®
is a combination of a monoclonal IgM (clone LDM3) and a monoclonal IgG antibody (clone ESD1).

Strong Partner

Both reagents fit very well together. They show comparable strong reactions with Rh-positive erythrocytes and react with a high percentage of D^weak samples. In the direct agglutination test (tube test with immediate spin or after incubation as well as plate test) both reagents do not detect category D^VI and therefore fulfill the requirements of the German guidelines¹ for patient testing.

For donor testing or for further clarification of discrepant results, Combi Anti-D Mono-Type^® can be further used in the indirect antiglobulin test. There, all D^weak as well as weak reacting D categories are securely captured.

The ideal combination for the D-determination

Rhesus D-determination for patients*
(i.e. potential receivers of a transfusion, pregnant women, newborns)

IgM Anti-D (MS-201) Mono-Type^®	Combi Anti-D Mono-Type^®	Autologous Serum	Results/Remark
pos.	pos.	neg.	Rh pos. (D pos.)
neg.	neg.	neg.	Rh neg. (D neg.)
pos. neg.	neg. pos.	neg. neg.	Further evaluation in specialized lab. In case of emergency, transfuse Rh neg. (D neg.).
neg./pos.	neg./pos.	pos.	Perform direct antiglobulin test-> Further evaluation in specialized lab. In case of emergency, transfuse Rh neg. (D neg.).

* Remarks:

–	Perform anti-D tests only as direct tests (no indirect antiglobulin test!)
–	For the statement “Rh pos. (D pos.)” both anti-D reagents need to react comparably strong positive. Consistent 1+ is sufficient. If the results are differing -> repeat the test (tube test according box insert is recommended). If the discrepancy is confirmed -> further evaluation in a specialized lab is recommended.

Comparative study using reagent red cells representing the different D categories

D-category	(Amount)	IgM Anti-D (MS-201) immediate spin*	Combi Anti-D immediate spin*	IAT
D^III	(5)	++++	++++	n.d.
D^IV	(4)	+++/++++	+++/++++	n.d.
D^Va	(2)	++++	++++	n.d.
D^VI	(13)	-	-	+/++/+++/++++
D^VII	(5)	+++/++++	+++/++++	n.d.
Rh33 Rh33	(10) (2)	++++ ++/+++	++++ +	n.d. +
DFR	(2)	-	±	+/++
DBT	(2)	+++/++++	++/+++	n.d.

* = tube test, immediate spin		IAT = indirect antiglobulin test
n.d. = not done

Literature

1.	Scientific council of the Bundesärztekammer: Guidelines for the preparation of blood and blood components and for the use of blood products (hemotherapy), Deutscher Aerzteverlag, Cologne (2000)
2.	Flegel, W.A., Northoff, H., Wagner, F.F.: New guidelines for the determination of blood groups and for blood transfusions, MTA 13 (1998)

Anti-C, -c, -E, -e Mono-Type^® (1) and new (2)
Our Rh-subgroup antisera are monoclonal antibodies, which are produced by heterohybridoma cell lines. All antisera have a low protein content, which facilitates the Rhesus determination in patients with positive direct antiglobulin test. As no high molecular weight polymers are added to the reagents, a special Rhesus control is not required.

Characteristics

	Strong and stable agglutinates in the tube test, on slide and in microtiter plates
	React at room temperature
	Low protein content requires no special Rhesus control reagent and facilitates the Rhesus subgroup typing in patients with positive direct antiglobulin test
	Different clones for the first and second clone line

Product list

top