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Enhancement Media
Enhancement media shorten the
incubation time and increase the
reaction strength in your cross
match and antibody screening.
Individual antibodies react optimally
under different conditions.
Some react best in
Albumin/Coombs, others react
better after the addition of
Enlisst® or Bromelase®.
Weak Rhesus antibodies react
much better in the presence of
Bromelase®.
Therefore, we recommend a combination of
techniques for the antibody screening.
A practical solution is to perform, in addition to
the Bromelase® test, the indirect antiglobulin
test for antibody screening in Albumin and the
cross match in Enlisst®.
The characteristic reaction mode of the individual
antibodies in different media can be used
to apply a combination of techniques for the
identification of antibodies.
Bromelase® 30
 | Proteolytic enzyme with special diluent |
| Lyophilized to ensure constant enzyme
activity |
| Economical: just one drop for each test |
| Especially suitable for the detection of Rhesus
antibodies |
Bromelase® 30 removes by its proteolytic activity
glycoproteins from the surface of erythrocytes.
| The negative surface potential is reduced and
therefore, the distance between individual
erythrocytes decreased. |
| Less accessible antigens are released. |
As a result, more antigen receptors are available
while incomplete antibodies are able to
agglutinate the red cells.
Bromelase® 30 is a lyophilized enzyme preparation
produced from pineapple.
A special diluent assures an optimal activity of
the reconstituted Bromelase for 30 days. The
lyophilized enzyme allows for transportation
and storage without any loss in activity.
Specific Bovine Albumin
| The classical enhancement medium for the
detection of IgG antibodies in the 3-step
Coombs test. |
| Available in polymerized (22% solution) or
monomeric (30% solution) form |
| US origin (BSE free) |
Enlisst®-II
| Modified LISS (Low Ionic Strength Solution)
with added 5% bovine albumin, gelatine and
glycine at an optimum pH of 6.7 |
| Intended for use as an enhancement medium
for the indirect antiglobulin test |
| Suitable for cross matching and antibody
screening |
| Only 2 drops needed per test |
| Reduces the incubation time to only 10–15
minutes |
Antigen–antibody reactions are positively influenced
by different factors. Low ionic strength
accelerates considerably the binding of antibodies
on the surface of erythrocytes. As an
example, the decrease of the ionic strength of
a physiological solution from 0.17 M to 0.03 M
results in a 1000-fold increase in the reaction
rate. Erythrocytes swell in hypotonic solution
due to water uptake. Therefore, antibodies
against cryptantigens are easier to detect under
such conditions. It is well known that the antigen–antibody reaction is pH dependent
and
that the optimum is at pH 6.7
High molecular substances like albumin reduce
the zeta potential and therefore reduce the
rejection of erythrocytes. The binding and
agglutination capability of IgG antibodies is
therefore increased.
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